LOCUS       sequence-111373-         238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pSB700
FEATURES             Location/Qualifiers
     source          1..238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     149..170
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aagcactgca cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
       61 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      121 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      181 accccggntg aacagctcct cgcccttgct cacnatggtg gccacaataa ccagcacg
//
