LOCUS       sequence-111374-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses codon optimized Cas9 in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pME-Cas9
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..128
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     130..146
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     156..175
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        156..174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      204..213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             222..242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
       61 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      121 agcaggcttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
      181 tgggtaccgg gccccccctc gacgccacca tggcttctcc acctaagaag aagagaaagg
      241 tgggaagcat ggataagaag tatagcatcg gcctggatat tggaactaac tccgtgggtt
      301 gggcagtgat tacagacgac tacaaggtcc ctagcaagaa atttaaggtg ctgggtaaca
      361 ccgacaggca cagcatcaag aaaaatctga ttggagccct gctgttcggt tctggagaga
      421 ctgccgaagc aacacgcctg aaaagaacag caagaaggcg ctataccaga aggaagaata
      481 gaatctgtta cctgcaggag attttctcta acgaaatggc taaggtggac gattcattct
      541 ttcataggct ggaggaaagt ttcctggtcg aggaagataa gaaacacgag cgccatccta
      601 tctttggaaa cattgtggac gaggtcgcct atcacgaaaa atacccaacc atctatcatc
      661 tgcgcaagaa actggctgac tctactgata aagccgacct gagactgatc tatctggctc
      721 tggcccacat gattaagttc aggggtcatt ttctgatcga gggcgatctg aaccccgaca
      781 attccgatgt ggacaagctg ttcatccagc tggtccagat ttacaatcag ctgtttgagg
      841 a
//
