LOCUS       sequence-111376-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA(tyr) under U6a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6a:sgRNA(tyr)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9..25
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        486..561
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc ggtctctcag taagtctctc
       61 agcgtttctc cagcctcggt cattcagtaa gacgagtttc ctgctctcgg cgtcttttgt
      121 tctggtcttc aaggaggggg gaaatgttct gcgcatgcct gtgggggagg gagaaggaca
      181 cgtcactgaa aacgtccctg catcacaccg agacacccaa tcactcaagc caagaccaga
      241 taattttgca tatgctttac agtttgaaaa ttaccacggt aatccctcac acaaactctg
      301 gatttgagat ccttcaggtt ttatcagttt gcaggtttat gtcaccatga tatagggtca
      361 gacttgatct aagggagctg aataagtggt ttagtcactc accacctccc aaaaacatac
      421 ccagaagtcc ctggtatata tagctctccc tccagctctt ggttcggact ggaggacttc
      481 tgggggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      541 aaagtggcac cgagtcggtg cttttttagt cagagacctc tagaccagcc aggacagaaa
      601 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
      661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
      721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggca
//
