LOCUS       sequence-111377-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Mathupala SP, Rempel A, Pedersen PL
  TITLE     Glucose catabolism in cancer cells: identification and 
            characterization of a marked activation response of the type II 
            hexokinase gene to hypoxic conditions.
  JOURNAL   J Biol Chem. 2001 Nov 16;276(46):43407-12. Epub 2001 Sep 13.
  PUBMED    11557773
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-11-16"; volume: "276(46)"; pages: "43407-12. 
            Epub 2001 Sep 13"
COMMENT     Sequence Label: pGL2-HKII-F
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    142..263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(179..198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     233..252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(748..766)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac
       61 gtggaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      241 tccaaactca tcaatgtatc ttatggtact gtaactgagc taacataacc cgggagagtg
      301 actctgataa ggtgcgatcg cccgagagga acagaactgt catttttgcg aagttgagcc
      361 ttacggatcc cgtgggcgaa gttagcgacg ggacgctgag caactagacc ggtcggcagg
      421 agtgagactt aggtgccttc tagtagttgt gacttaaaaa aaaaaaaaaa aggaaaagaa
      481 aaaaggagga aaacctgttt ctggaaacgc gaggccctca gctggtgagc catcgtggtt
      541 aagcttcttt gtgtggctcc tggagtctcc gatcccagcc ggacacccgg gcctggtttc
      601 aaagcggtcg aactgctctg cccgctccac cggtagcgct cgagatctaa gtaagcttgg
      661 cattccggta ctgttggtaa aatggaagac gccaaaaaca taaagaaagg cccggcgcca
      721 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
      781 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
      841 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
      901 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
      961 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     1021 ttgctcaaca g
//
