LOCUS       sequence-111379-        3909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Ssf1 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Ssf1
FEATURES             Location/Qualifiers
     source          1..3909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2034..2133)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2150..2169)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2151..2169)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2174..2190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2174..2190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2292..2311)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2303..3112
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2370..2389)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3259..3847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3748..3767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatggcca agagaagaca aaagaaaaga acacatgcac agcttacacc
      721 tgagcaggaa caaggtatac ctaagtcaat ggtaatcaga gtgggtcaaa cttctttggc
      781 caaccattct ctgaaccaat tagtaaagga cttccgccaa atcatgcaac cacatacggc
      841 tataaaattg aaagagcgca agtccaataa actgaaggat ttcgttgtta tgtgtggccc
      901 attaggtgtt acgcatcttt ttatgttcac tcaatctgaa aaaacaggaa atgtctccct
      961 aaagatagct agaactccgc aaggtcctac tgttactttt caagtattgg actattctct
     1021 gggtagagat atcaaaaaat ttttgaagag acctaaatcg ttgaataatg atgatgtatt
     1081 aaatcctcca ttgctagtgc taaacgggtt ttccacatcc aaaagatctg gtgaagacga
     1141 ccaggatgta aacgtggaga aggtcattgt ttctatgttt cagaatattt tcccacctct
     1201 gaatcctgcg agaacttctt tgaattctat caaacgtgta tttatgatca ataaagacag
     1261 ggaaacaggg gaaatttcta tgcgccacta ttttattgac attcgagaag tagagatctc
     1321 tagaaaccta aaaaggcttt ataaagccaa aaataatcta agtaaaacag tgccgaattt
     1381 gcatcgaaaa gaagatattt cttctttgat attagatcac gacttaggcg cctatacatc
     1441 agaatctgaa attgaagatg acgctatcgt gagagtagtt gataatcagg acgtgaaggc
     1501 aaagcattct caaagtttga agtcccaaag aacaccggtc gaaaagaagg ataataaaga
     1561 gcgtgaaaaa gaaacagaag aagaagatgt ggaaatggag gaacctaagc cttcagaaaa
     1621 cttgcaacca accccacgta agaaagctat caaactaact gaattaggac caagattaac
     1681 tttaaaactg gtcaagatag aagaaggtat ttgttcaggt aaagtgttac atcatgaatt
     1741 tgttcaaaaa tcaagcgaag aaatcaaagc tttagaaaag agacacgctg caaaaatgag
     1801 gctgaaggaa caaagaaaga aggaacaaga agaaaacatt gctaaaaaga aggctgttaa
     1861 agacgctaaa aagcaacgta aattggaaag aagaaaagct agagcagcag aaggaggtga
     1921 aggtcaagga aaagatgatg cgatgagcga tgatgagtct tcatcaagtg acagcgaaca
     1981 ttatggtagc gtaccagagg atctagatag tgacttattt agtgaggtcg aaaacccagc
     2041 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2101 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2161 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2221 atgttacatt gcacaagata aaataatatc atcatgaaca ataaaactgt ctgcttacat
     2281 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     2341 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2401 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2461 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2521 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2581 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2641 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2701 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2761 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2821 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2881 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2941 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     3001 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3061 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     3121 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     3181 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3241 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3301 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3361 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3421 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3481 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3541 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3601 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3661 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3721 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3781 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3841 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3901 tcacatgtt
//
