LOCUS       sequence-111385-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for two U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-2sgRNA
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..259)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(267..283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 actttattat acatagttga taattcactg gccgtcgttt tacggtacgc taggtctctc
      301 tcagtaccat taatagtgtg cattcagtgc aggatgttat gtatttattt tctgtagtgc
      361 aaactttttc aaagaatttt ccagtctaag cttttcagtc aagaaaaaaa aaaagcgatt
      421 gattatctgt gatatttaaa aaaattagct cttaaatctg gtgaaccaag tggccacaac
      481 acattaaata ctgacgttct acagccatgt agcttcatct ggtctggttt gttttggcag
      541 gcacttattt tccggtgata gttggccaca agagtattgt ctgagccatt cagtgctaga
      601 ctgtcattct caggtcagag tccaccccgc tgattctgct gacaacactg ttcccaccat
      661 gagataatgc cattccagag agatccattt gtaagcccct ctttctgcag cacaggtata
      721 taaccagggg tctgcctcca ctaaggccgg caca
//
