LOCUS       sequence-111404-        1721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD F447S-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS532 (TJF075)
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1693..1710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgaagaagg tttggcttaa ccgttatccc gcggacgttc cgacggagat caaccctgac
       61 cgttatcaat ctctggtaga tatgtttgag cagtcggtcg cgcgctacgc cgatcaacct
      121 gcgtttgtga atatggggga ggtaatgacc ttccgcaagc tggaagaacg cagtcgcgcg
      181 tttgccgctt atttgcaaca agggttgggg ctgaagaaag gcgatcgcgt tgcgttgatg
      241 atgcctaatt tattgcaata tccggtggcg ctgtttggca ttttgcgtgc cgggatgatc
      301 gtcgtaaacg ttaacccgtt gtataccccg cgtgagctag agcatcagct taacgatagc
      361 ggcgcatcgg cgattgttat cgtgtctaac tttgctcaca cactggaaaa agtggttgat
      421 aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg atcagctatc tacggcaaaa
      481 ggcacggtag tcaatttcgt tgttaaatac atcaagcgtt tggtgccgaa ataccatctg
      541 ccagatgcca tttcatttcg tagcgcactg cataacggct accggatgca gtacgtcaaa
      601 cccgaactgg tgccggaaga tttagctttt ctgcaataca ccggcggcac cactggtgtg
      661 gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga acctggaaca ggttaacgcg
      721 acctatggtc cgctgttgca tccgggcaaa gagctggtgg tgacggcgct gccgctgtat
      781 cacatttttg ccctgaccat taactgcctg ctgtttatcg aactgggtgg gcagaacctg
      841 cttatcacta acccgcgcga tattccaggg ttggtaaaag agttagcgaa atatccgttt
      901 accgctatca cgggcgttaa caccttgttc aatgcgttgc tgaacaataa agagttccag
      961 cagctggatt tctccagtct gcatctttcc gcaggcggag ggatgccagt gcagcaagtg
     1021 gtggcagagc gttgggtgaa actgacagga cagtatctgc tggaaggcta tggccttacc
     1081 gagtgtgcgc cgctggtcag cgttaaccca tatgatattg attatcatag tggaagcatc
     1141 ggtttgccgg tgccgtcgac ggaagccaaa ctggtggatg atgatgataa tgaagtacca
     1201 ccgggtcaac cgggtgagct ttgtgtcaaa gggccgcagg tgatgctggg ttactggcag
     1261 cgtccggatg ctacagatga gatcatcaaa aatggctggt tacacaccgg cgacatcgcg
     1321 gtgatggatg aagaaggatc cctgcgcatt gtcgatcgta aaaaagacat gattctggtt
     1381 tccggtttta acgtctatcc caacgagatt gaagatgtcg tcatgcagca tcccggcgta
     1441 caggaagtcg cggctgttgg cgtaccttcc ggctccagtg gtgaagcggt gaaaatcttc
     1501 gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg tgaccttttg ccgccgtcag
     1561 ctcacgggct acaaagtacc gaagctggtg gagtttcgtg atgagttacc gaaatctaac
     1621 gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc gcggcaaagt ggacaataaa
     1681 gccggttctt ctcatcacca tcatcatcac tgaatgaatg a
//
