LOCUS       sequence-111409-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG225
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     139..155
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     140..156
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(190..206)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(190..206)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(314..333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(315..333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(340..357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(340..356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(349..371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        567..972
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctgggtacaa
       61 gtgacaatat atatatatat atataataac gacatcgtta tctgtaaata cggccgaagc
      121 tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc
      181 agcccggggg atccactagt tctagagcca ccgcggtgga gctccaattc gccctatagg
      241 cggccgcgaa aagaaatata gtcttcaagg ttatattata caggtttata tattattctg
      301 tttctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
      361 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atccgatcga
      421 aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa aaacgttata tttataggat
      481 aattatactc tatttctcaa caagtaattg gttgtttggc cgagcggtct aaggcgcctg
      541 attcaagaaa tatcttgacc gcagttaact gtgggaatac tcaggtatcg taagatgcaa
      601 gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg
      661 cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc
      721 tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg
      781 aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact
      841 tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt
      901 cttactttct aacttttctt accttttaca tttcagcaat atatatatat atttcaagga
      961 tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
     1021 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
//
