LOCUS       sequence-111421-        9724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP32
FEATURES             Location/Qualifiers
     source          1..9724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             465..487
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             507..524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             528..560
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(564..572,573..587)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    1302..1426
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1451..1456,1457..1474,1475..1481)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1601..1620)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2594..2899
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2778..2797
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2940..3064)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3102..3123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3363..3382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3710..3731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3747..3776
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3814..3830)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3815..3831)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3855..3874)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3856..3874)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      4022..4209
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4077..4101)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4221..4238)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4221..4237)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4230..4252)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4673..6019
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6130..6933)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6934..7149)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7174..7192)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7260..7364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7365..7433,7434..8225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7583..7602)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8396..8984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8885..8904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9138..9155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9272..9293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9308..9313,9314..9331,9332..9338)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9346..9362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9351..9373
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9370..9386
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9370..9386
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
       61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
      121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
      181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
      241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
      301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
      361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
      421 aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt taactttaag
      481 aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
      541 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgggcctcg
      601 aggtttctaa aggtgaagaa ttgtttacgg gcgtcgtccc gatcctcgtg gaactcgacg
      661 gggatgttaa cgggcataag ttttcggtca gcggggaagg ggagggggac gcgacgtatg
      721 ggaagctcac tctcaagctg atctgtacga cggggaaact cccggtcccg tggccgacgc
      781 tggtcacgac gctgggatac gggctccaat gctttgcgag gtatccggac cacatgaaac
      841 agcatgactt tttcaaatcg gcgatgccgg agggatacgt gcaggaacgg acgatctttt
      901 tcaaagacga tgggaactat aagacgcggg cggaagtcaa gtttgaaggg gacacgctcg
      961 tcaaccggat cgaactcaag gggattgact tcaaagagga tgggaacata ctcggccata
     1021 agctcgaata caattacaac tcgcataacg tatacatcac cgcggataag caaaagaacg
     1081 ggatcaaagc caatttcaaa atccggcata acatagagga tgggggggtc caactggcgg
     1141 atcactatca gcaaaacacg ccgatagggg atgggccggt cctcctcccg gataaccatt
     1201 acctctcgta ccaaagcgcg ctctcgaagg acccgaatga gaaacgggac cacatggttc
     1261 tcctggagtt cgtcacggcg gcgggcatag gtaccgatat cacaagtttg tacaaaaaag
     1321 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     1381 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     1441 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1501 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1561 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1621 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1681 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1741 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1801 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1861 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1921 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1981 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2041 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2101 tcaggttcat catgccgttt gtgatgggct tccatgtcgg cagaatgctt aatgaattac
     2161 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     2221 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2281 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2341 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2401 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2461 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2521 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaacttttgc tgacgagaac
     2581 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     2641 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     2701 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     2761 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     2821 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     2881 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     2941 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     3001 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     3061 tggtgatatc gactagtctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3241 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3481 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     3541 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3661 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3721 tggagttcgt gaccgccgcc gggatcgaac aaaaattgat aagtgaggaa gatttataag
     3781 ggccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
     3841 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg gatccagctt tggacttctt
     3901 cgccagaggt ttggtcaagt ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat
     3961 ttacgaaaag atggaaaagg gtcaaatcgt tggtagatac gttgttgaca cttctaaata
     4021 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     4081 atacaaattt taaagtgact cttaggtttt aaaacgaraa ttcttattct tgagtaactc
     4141 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     4201 ctctaccggc atgccaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4261 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4321 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     4381 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat aatcggatcg
     4441 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
     4501 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
     4561 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
     4621 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
     4681 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     4741 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     4801 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
     4861 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
     4921 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
     4981 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
     5041 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
     5101 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
     5161 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
     5221 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
     5281 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
     5341 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
     5401 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     5461 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
     5521 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
     5581 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
     5641 tcctatactt tctagctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga
     5701 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg
     5761 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg
     5821 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta
     5881 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct
     5941 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca
     6001 tttcctttga tattggatcg atccgatgat aagctgtcaa acatgagaat tgggtaataa
     6061 ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa
     6121 tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct
     6181 gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat
     6241 aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc
     6301 gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc
     6361 tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc
     6421 aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc
     6481 tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc
     6541 gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat
     6601 tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct
     6661 gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc
     6721 ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc
     6781 taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa
     6841 gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt
     6901 agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc tgcatgtttt
     6961 tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat
     7021 gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat
     7081 taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga
     7141 tgaattgaaa agctaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     7201 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7261 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7381 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7441 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7501 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7561 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7621 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7681 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7741 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7801 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7861 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7921 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7981 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8041 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8101 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8161 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8221 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8281 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8341 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8401 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8461 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8521 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8581 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8641 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8701 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8761 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8821 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8881 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8941 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9001 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9061 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9241 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9301 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9361 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttcctgaa acggagaaac
     9421 ataaacaggc attgctggga tcacccatac atcactctgt tttgcctgac cttttccggt
     9481 aatttgaaaa caaacccggt ctcgaagcgg agatccggcg ataattaccg cagaaataaa
     9541 cccatacacg agacgtagaa ccagccgcac atggccggag aaactcctgc gagaatttcg
     9601 taaactcgcg cgcattgcat ctgtatttcc taatgcggca cttccaggcc tcgatcgaga
     9661 ccgtttatcc attgcttttt tgttgtcttt ttccctcgtt cacagaaagt ctgaagaagc
     9721 tata
//
