LOCUS       sequence-111455-        4806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Drs1 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Drs1
FEATURES             Location/Qualifiers
     source          1..4806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2931..3030)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3047..3066)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3048..3066)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3071..3087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3071..3087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3189..3208)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3200..4009
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3267..3286)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4156..4744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4645..4664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatggtgg taggaactaa aaaatactct aatttggact ttgtccctac
      721 aatcagtgac agtgaagacg atgttccaat tctagattct tctgatgacg aaaaagtcga
      781 ggctaagaag actacgaaga agcggaaggg taagaataac aagaaaaagg ttagtgaggg
      841 ggataacctc gatgaggatg ttcatgagga cttggacgcg gggtttaagt ttgatttgga
      901 cgccgatgat accacttcga acttccaagg ctggaacttt ctagcagagg gcgagtccaa
      961 taaggacgat gccgaagctt ttgtgaagaa ggacgttgac ttggataaga ttattagaag
     1021 aaaaggtggg ctggtgaaaa tggcccatat tgatagtaaa caagaagaag aaaccgagaa
     1081 agaaaaagta gaaaaagaaa acgatagcga cgatgaggaa ttagcaatgg acgggttcgg
     1141 tatgggagct cccatgaaca atggagacga aaatcagtca gaagaagaag aagaagagga
     1201 ggaaaaggaa gaggaagagg aggaagagga ggaacaagaa gagatgacgt tagaaaaagg
     1261 cggcaaagat gacgaaatag atgaagaaga cgattctgaa gaggcaaaag ccgatttcta
     1321 tgcgcctgaa actgagggag atgaagctaa aaagcaaatg tacgaaaatt tcaacagttt
     1381 gtctttatct cgtccggttc ttaagggcct tgcaagtttg ggttacgtca agccttcccc
     1441 tattcaaagc gccacaatcc ccattgcctt attgggtaaa gacatcattg ccggtgctgt
     1501 gactggttcc ggtaagactg ctgcgtttat gattcccata atcgagcgtt tgttgtataa
     1561 accagccaaa atcgcttcca ccagagttat tgttctattg cccactcgtg agttagctat
     1621 ccaagtcgct gacgttggta aacaaattgc acgtttcgtc tccggtataa cctttggtct
     1681 ggccgttggt ggtttgaact tgagacaaca agaacaaatg ttgaaatctc gtccggacat
     1741 cgtcattgct accccaggta gattcattga tcatatcagg aactcagcaa gttttaatgt
     1801 ggactcagta gagattctgg ttatggatga agccgataga atgttagaag aaggttttca
     1861 agatgaactg aacgaaatta tgggcctatt accaagcaat agacagaacc tattgttttc
     1921 tgctacaatg aactccaaaa ttaaaagttt agttagtctt tctctaaaaa aaccagtaag
     1981 gattatgatt gatcctccaa agaaagctgc tactaagttg acacaagaat tcgttcgtat
     2041 tcgtaaaaga gaccatttga agcctgcctt gttatttaat ttgattagga aattggatcc
     2101 aacgggtcaa aagaggattg tcgtttttgt ggctagaaaa gaaactgctc ataggttaag
     2161 gattatcatg ggtcttttag gtatgagtgt gggtgaatta cacggttctt taacccaaga
     2221 acagcgttta gattccgtta ataaattcaa aaatttggaa gttcctgtac ttatctgtac
     2281 ggatttggcc tccagaggtc ttgatatccc caagattgag gttgttatca actacgatat
     2341 gcccaagagt tatgagatct acctgcatag agttggtcgt accgccagag ctggtaggga
     2401 aggtcgttcc gtcaccttcg tcggtgaatc atctcaagat agaagtattg tacgtgctgc
     2461 tataaagagt gtagaagaaa ataagtccct aactcaaggt aaagcacttg gtagaaacgt
     2521 agactgggtt caaatcgaag aaacaaacaa acttgttgaa tccatgaacg atacgattga
     2581 agatattctg gtggaagaaa aggaggagaa ggaaatatta agggctgaaa tgcaattaag
     2641 aaagggtgaa aatatgttga agcataaaaa ggaaatccag gcaagaccaa gaaggacatg
     2701 gttccaaagc gaatcagata agaaaaattc caaagtatta ggtgctttat caaggaacaa
     2761 gaaagtcact aacagcaaaa agagaaagcg tgaagaagct aaggcagatg gcaatggtgc
     2821 acgttcttat agaaaaacca aaaccgaccg tattgcagat caagaaagaa cttttaaaaa
     2881 gcagaagagt acaaattcaa ataagaagaa gggcttcaaa agccgtagga acccagcttt
     2941 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     3001 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     3061 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     3121 ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa
     3181 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3241 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     3301 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3361 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3421 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3481 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3541 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3601 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3661 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3721 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3781 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3841 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3901 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3961 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     4021 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     4081 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     4141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4801 catgtt
//
