LOCUS       sequence-111458-        4446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Grc3 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Grc3
FEATURES             Location/Qualifiers
     source          1..4446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2571..2670)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2687..2706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2688..2706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2711..2727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2711..2727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2829..2848)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2840..3649
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2907..2926)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3796..4384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4285..4304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatggtga tagattccaa acaggatcta cctcaatata ccaaagattc
      721 aggttcagaa tcggactcag actcaagtaa taatttcatt gtggaatctc cgtcaattcc
      781 atcttcaaaa agtgcaaccg tagttctcaa tagtgaagag tatgaggacg acgaaggtga
      841 tgacttgaac gggctggatg ctgaattgat cgataatata acgtatgaag gagatgaaga
      901 tgaaactatg tttgtggggt tgaaagaaaa acagaagtta catttatctg gtgttttccg
      961 gctgcaagtg gtgaaaggag gtattgtcta caacaatgta cactataacg cttcgagaga
     1021 aattttaacc ttctggcacc cgttatcaca gtccatacct actatagatt tttcgcactt
     1081 tgcaggctgg caagacactt tttttatgcc gagaaacaac aggtttaaaa ttagagatga
     1141 agaattcaaa tcatttccat gtgttcttcg cgtttttaat tctaatcata cgggcttgtt
     1201 agaagcaggt catttatatc gggatgttaa ttatctatgg aaacctaaag aaccttactt
     1261 tccattaaac gagagaacaa cgtatcattt attacatgag tcagaccgaa ttcaatcttt
     1321 atccgtgccg ggatattggt caactccgtt agagaagtta tatttgagtc acaagaacgc
     1381 tgcgtatgat acaagaatca tggtgattgg tggtaaaaac tctgggaagt ctacattctt
     1441 gaggctttta ttggaaaaat ttacgcaaga tatacgagat tctactacga gccaggaaga
     1501 attggtatac ctggatttag atcctggtca acctgaatac tcactacctg attcaatatc
     1561 cctgaataag attctttcca gccctatttc tctcggacaa catctctgcc aaggttcaaa
     1621 tttccaaact ttgttacagt tctacgctgg ctcctcttcc ccgcaggacg agcccacatc
     1681 atacctgaac tgcgctgata aacttataga ccatttggaa gagcaagcct tcttcggaac
     1741 gtcattgctt aatctacctg ggtggataaa aggattcggg atgcagattt taaatcatat
     1801 aataaggaaa tataagccca ctcacttatt gtttttggaa acagctaatt cgaaaagaca
     1861 cctggatgaa ctaacaattc cacagagttt ttccacatca ctacgagatg catatgcacc
     1921 tgaagttgtt cgcgttccag cccatagtct gaaccacacc ttatcttcta ggttccatgc
     1981 atcacaactg agaacattca aaatactggc actgtttcat aaaataactc aatttgatta
     2041 tgattttgcg cctcttttaa aatctgcacc tttgcaaatt tcatatggca aaggtaaaag
     2101 cggtattaaa ggcattcaat ttcctatgga attccaggat ctgaatcctc aagatatcaa
     2161 gtccgcattg gaaggtactg tcattgggat atatacatat tctggagaag actcactaga
     2221 ggtgaaatcc ttgaatacct ttcccatcct acaatcgtgt acttcttctt ctaaaaattt
     2281 cataactctg gggctgatac actctattga cacctcccag caaataatga acatttacgt
     2341 gccaccttgc catacccaga ttttagataa acaacctgag gatgctcaat ggataatagt
     2401 aagaaataag acggaaactc ctttttgtga ctttcttcca tccccccgca caattacatg
     2461 ggatgataat atacaaatac cgttcgcaac ttttgaaaga aggaaaaaat tggaacatgt
     2521 gtggaaagtt cgtaagaacg tcatgagacg tggccaattc atgaaaagaa acccagcttt
     2581 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2641 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2701 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2761 ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa
     2821 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2881 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2941 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3001 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3061 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3121 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3181 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3241 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3301 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3361 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3421 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3481 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3541 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3601 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3661 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3721 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3901 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4441 catgtt
//
