LOCUS       sequence-111460-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for five U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-5sgRNA
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(79..85,86..103,104..109))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(134..257)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(265..281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
       61 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
      121 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
      181 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
      241 tttattatac atagttgata attcactggc cgtcgtttta cggtacgcta ggtctctgtt
      301 cgtaccatta atagtgtgca ttcagtgcag gatgttatgt atttattttc tgtagtgcaa
      361 actttttcaa agaattttcc agtctaagct tttcagtcaa gaaaaaaaaa aagcgattga
      421 ttatctgtga tatttaaaaa aattagctct taaatctggt gaaccaagtg gccacaacac
      481 attaaatact gacgttctac agccatgtag cttcatctgg tctggtttgt tttggcaggc
      541 acttattttc cggtgatagt tggccacaag agtattgtct gagccattca gtgctagact
      601 gtcattctca ggtcagagtc caccccgctg attctgctga caacactgtt cccaccatga
      661 gataatgcca ttccagagag atccatttgt aagcccctct ttctgcagca caggtatata
      721 accaggggtc tgcctccact aaggccggca cacatcattt ggggatcttt gtactgtact
      781 agcctcta
//
