LOCUS       sequence-111462-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for one U6x:sgRNA cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-1sgRNA
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(80..86,87..104,105..110))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(135..258)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(266..282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
       61 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
      121 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa
      241 ctttattata catagttgat aattcactgg ccgtcgtttt acggtacgct agcggtctct
      301 agtcggtacc attaatagtg tgcattcagt gcaggatgtt atgtatttat tttctgtagt
      361 gcaaactttt tcaaagaatt ttccagtcta agcttttcag tcaagaaaaa aaaaaagcga
      421 ttgattatct gtgatattta aaaaaattag ctcttaaatc tggtgaacca agtggccaca
      481 acacattaaa tactgacgtt ctacagccat gtagcttcat ctggtctggt ttgttttggc
      541 aggcacttat tttccggtga tagttggcca caagagtatt gtctgagcca ttcagtgcta
      601 gactgtcatt ctcaggtcag agtccacccc gctgattctg ctgacaacac tgttcccacc
      661 atgagataat gccattccag agagatccat ttgtaagccc ctctt
//
