LOCUS       sequence-111464-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for four U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-4sgRNA
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(71..77,78..95,96..101))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(126..249)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(257..273)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg atcctaactc
       61 aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt gcctaatgcg
      121 gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      181 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca actttattat
      241 acatagttga taattcactg gccgtcgttt tacggtacgc taggtctctc ctggtaccat
      301 taatagtgtg cattcagtgc aggatgttat gtatttattt tctgtagtgc aaactttttc
      361 aaagaatttt ccagtctaag cttttcagtc aagaaaaaaa aaaagcgatt gattatctgt
      421 gatatttaaa aaaattagct cttaaatctg gtgaaccaag tggccacaac acattaaata
      481 ctgacgttct acagccatgt agcttcatct ggtctggttt gttttggcag gcacttattt
      541 tccggtgata gttggccaca agagtattgt ctgagccatt cagtgctaga ctgtcattct
      601 caggtcagag tccaccccgc tgattctgct gacaacactg ttcccaccat gagataatgc
      661 cattccagag agatccattt gtaagcccct ctttctgcag cacaggtata taaccagggg
      721 tctgcctcca ctaaggccgg cacacatcat ttggggatct ttgtactgta ct
//
