LOCUS       sequence-111465-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for three U6x:sgRNA cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pGGDestTol2LC-3sgRNA
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..270)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(278..294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacg
      301 ctaggtctct ccaagtacca ttaatagtgt gcattcagtg caggatgtta tgtatttatt
      361 ttctgtagtg caaacttttt caaagaattt tccagtctaa gcttttcagt caagaaaaaa
      421 aaaaagcgat tgattatctg tgatatttaa aaaaattagc tcttaaatct ggtgaaccaa
      481 gtggccacaa cacattaaat actgacgttc tacagccatg tagcttcatc tggtctggtt
      541 tgttttggca ggcacttatt ttccggtgat agttggccac aagagtattg tctgagccat
      601 tcagtgctag actgtcattc tcaggtcaga gtccaccccg ctgattctgc tgacaacact
      661 gttcccacca tgagataatg ccattccaga gag
//
