LOCUS       sequence-111466-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast expression vector p415GPD modified such that it is Gateway 
            converted and introduces a 3xHA tag at the N-terminus of the insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: p415GPD-3xHA-GW
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..167
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    171..295
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(320..325,326..343,344..350)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(470..489)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga tctcatgtac ccatacgatg ttcctgacta tgcgggctat ccctatgacg
      121 tcccggacta tgcaggatcc tatccatatg acgttccaga ttacgctggc acaagtttgt
      181 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      241 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
      301 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
      361 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      421 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      481 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
      541 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
      601 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
      661 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
      721 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
      781 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
      841 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
      901 cttcgccccc gttttc
//
