LOCUS       sequence-111467-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6c promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6c:sgRNA#4
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(625..642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(625..641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(634..656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggtctagag
       61 gtctctcagg tttaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
      121 agccttattt aaacttgcta tgctgtttcc agcatagctc ttaaacagag acggtcgaca
      181 gtctgcagtg tcgtctctcg aactaggagc ctggagaact gctatataaa cccgggactt
      241 ctgggtatgt ttctgccatg tggtaagtgg ataaacctac ttattcaact catttacctc
      301 aaacatgaca gtaaacctct tcggggcaaa cctgcaaacg cttaaaacct tcaaagtttg
      361 aaaccagcag ttttgtagag gtttaccgta gtatttttga actgtaaagc atatgcagag
      421 gaagctggtt ttaggcttgt gtgattggat gagttcttct gtgacgtaac tgatcttttg
      481 tcctcgtgtc cttcgccttc ccccacaggc atgcgcggac cttcccccgc cgcagaccag
      541 aggacaacat gctcagacgc cagcaaacgg cccatcgaaa cattcacaga ctctcggatt
      601 ggagagaccg ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
      661 catccatgct agcgttaacg cgagagtagg gaactgccag gcatc
//
