LOCUS       sequence-111470-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6a:sgRNA#2
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(636..653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(636..652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(645..667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccggcaacct tgggtagcag cgaagtcgag gcatttctgt cctggctggt ctagaggtct
       61 cttgagttta aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
      121 ttatttaaac ttgctatgct gtttccagca tagctcttaa acagagacgg tcgacagtct
      181 gcagtgtcgt ctctcgaacc aagagctgga gggagagcta tatataccag ggacttctgg
      241 gtatgttttt gggaggtggt gagtgactaa accacttatt cagctccctt agatcaagtc
      301 tgaccctata tcatggtgac ataaacctgc aaactgataa aacctgaagg atctcaaatc
      361 cagagtttgt gtgagggatt accgtggtaa ttttcaaact gtaaagcata tgcaaaatta
      421 tctggtcttg gcttgagtga ttgggtgtct cggtgtgatg cagggacgtt ttcagtgacg
      481 tgtccttctc cctcccccac aggcatgcgc agaacatttc ccccctcctt gaagaccaga
      541 acaaaagacg ccgagagcag gaaactcgtc ttactgaatg accgaggctg gagaaacgct
      601 gagagacttg actagagacc gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt
      661 gactgggaaa
//
