LOCUS       sequence-111471-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG213MX
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..139
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     123..139
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(173..189)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(174..190)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(288..306)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(327..343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(327..343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(340..362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    351..367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(601..618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(852..871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tggagaaaca gaataatata taaacctgta taatataacc
       61 ttgaagacta tatttctttt cgcggccgcc tatagggcga attggagctc caccgcggtg
      121 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
      181 tcgacctcga gggggggccc ggtacccagc ttcggccgta tttacagata acgatgtcgt
      241 tattatatat atatatatat attgtcactt gtacccagct tttgttccct ttagtgaggg
      301 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      361 ctcacaacag ctgacaccga ttatttaaag ctgcagcata cgatatatat acatgtgtat
      421 atatgtatac ctatgaatgt cagtaagtat gtatacgaac agtatgatac tgaagatgac
      481 aaggtaatgc atcattctat acgtgtcatt ctgaacgagg cgcgcctctc cctgcaggca
      541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1021 ctgt
//
