LOCUS       sequence-111475-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast expression vector p415GPD modified such that it is Gateway 
            converted and introduces a 3xHA tag at the N-terminus of the insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: p415GPD-3xHA-GW
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(7..196)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     208..226
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    245..369
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(410..715)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(512..531)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa tgttacatgc
       61 gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg ttcttaatac
      121 taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa ctccttcctt
      181 ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa ctaattacat
      241 gactaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
      301 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      361 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
      421 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
      481 cttccccgat aacggaaacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
      541 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
      601 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
      661 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
      721 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
      781 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
      841 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc a
//
