LOCUS       sequence-111482-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses destabilized Ametrine in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: FRP1483
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(205..454)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     420..438
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtacca agcagacgct actaaggaaa acaacaacgg aagcgatgat gagagcgacg
      181 atgagagtgt aaactgcgaa ccttgcaaat taaagccttc gagcgtccca aaaccttctc
      241 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa
      301 aatttgaaat ataaataacg ttcttaatac taacataact attaaaaaaa ataaataggg
      361 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt gggaggaggg
      421 cgtgaatgta agcgtgacat aactaattac atgatataga caaaggaaaa ggggcctgtc
      481 tcgagttatt tagaagtgtc aacaacgtat ctaccaacga tttgaccctt ttccatcttt
      541 tcgtaaattt ctggcaaggt agacaagccg acaaccttga ttggagactt gaccaaacct
      601 ctggcgaaga agtccaaagc tctgaattga gtttccaaac cggatcttct aacagtagac
      661 caagtggatc ctttgtataa ttcatccata cctggagtaa taccagcagc agtaacaaat
      721 tccaacaaga ccatgtgatc tctttgttcg tttgggtcct tgaacaactt ggattggaag
      781 gacaagtaat ggttatctgg caacaaaact gaaccatcac caattggggt gttttgttga
      841 taagcatcag ccaattgtac accaccatct tcaacattgt gttcgatctt gaagttggcc
      901 ttaataccgt tcttttgctt atcagcggta atgtaagcat cccaaacgtt catgttgtat
      961 tccaacttat gacccaagat gttaccatct tctttgaagt cgataccctt caattcgatt
     1021 ctgttaacca aggtatcacc ttcgaactta acttcggctc tagttctata agaaccgtca
     1081 tccttgaa
//
