LOCUS       sequence-111489-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG227
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      4..986
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(967..1014)
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     protein_bind    complement(967..1014)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtcaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
       61 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
      121 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
      181 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
      241 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
      301 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
      361 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
      421 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
      481 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
      541 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
      601 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
      661 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
      721 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
      781 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
      841 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
      901 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
      961 cgctctgaag ttcctatact ttttagagaa taggaacttc ggaataggaa cttcaaagcg
     1021 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     1081 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatga
//
