LOCUS       sequence-111490-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG227
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     140..156
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     141..157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(191..207)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(315..334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(341..358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(341..357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(350..372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        474..713
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
      181 cagcccgggg gatccactag ttctagagcc accgcggtgg agctccaatt cgccctatag
      241 gcggccgcga aaagaaatat agtcttcaag gttatattat acaggtttat atattattct
      301 gtttctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccgatcg
      421 agaggcatcg cacagtttta gcgaggaaaa ctcttcaata gttttgccag cggaattgca
      481 cttgcaatta cataaaaaat tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct
      541 gcctaatgaa catgaacatc gcccaaatgt atttgaagac ccgctgggag aagttcaaga
      601 tatataagta acaagcagcc aatagtataa aaaaaaatct gagtttatta cctttcctgg
      661 aatttcagtg aaaaactgct aattatagag acatatcaca gagttactca ctaatgacta
      721 acgaaaaggt ctggatagag aagttggata atccaactct ttcagtgtta ccacatgact
      781 ttttacgccc acaacaagaa ccttatacga aacaagctac atattcgtta cagctacctc
      841 agctcgatgt gcctcatgat agtttttcta acaaatacgc tgtcgctttg agtgtatggg
      901 ctgcattgat atatagagta actggtgacg atgatattgt tctttatatt gcgaataaca
      961 aaatcttaag attcaatatt caaccaacgt ggtcatttaa tgagctgtat tctacaatta
     1021 acaatgagtt gaacaagctc aattctattg aggccaattt t
//
