LOCUS       sequence-111492-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA-ER-B42 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: FRP1625
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(205..454)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     420..438
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     481..497
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     482..498
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(531..767)
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /label=B42 transcriptional activator
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
ORIGIN
        1 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggtaca
       61 agtgacaata tatatatata tatataataa cgacatcgtt atctgtaaat acggccgaag
      121 ctgggtacca agcagacgct actaaggaaa acaacaacgg aagcgatgat gagagcgacg
      181 atgagagtgt aaactgcgaa ccttgcaaat taaagccttc gagcgtccca aaaccttctc
      241 aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa
      301 aatttgaaat ataaataacg ttcttaatac taacataact attaaaaaaa ataaataggg
      361 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt gggaggaggg
      421 cgtgaatgta agcgtgacat aactaattac atgatataga caaaggaaaa ggggcctgtc
      481 tcgaggtcga cggtatcgat aagctttaaa cgcgtttaaa gcttggaggc atctccactc
      541 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
      601 cagtcaggct ggatcggcgg cccggcgtga agaatggttt tcggcgtcat gcccggcacc
      661 acgttaatcg cctgatccgc catttccatc ggcatctcct gtccggtgcg caggtagtcg
      721 ataaactgct caatgatggc attgcactcc tcgatgtctt tattgatacc gcccgcggtg
      781 ctgttatcgt tgcccggcag atcctgcatg atatcgactg tggcagggaa accctctgcc
      841 tcccccgtga tgtaatactt ttgcaaggaa tgcgatgaag tagagcccgc agtggccaag
      901 tggctttggt ccgtctcctc cacggatgcc cctccacggc tagtgggcgc atgtaggcgg
      961 tgggcgtcca gcatctccag cagcaggtca tagaggggca ccacgttctt gcacttcatg
     1021 ctgtacagat gctccatgcc tttgttactc atgtgcctga tgtgg
//
