LOCUS       sequence-111493-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA-ER-B42 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: FRP1625
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gaaacagaat aatatataaa cctgtataat
       61 ataaccttga agactatatt tcttttcgcg gccgcctata gggcgaattg gagctccacc
      121 gcggtggcgg ccgctctaga gaagcgggta agctgccaca gcaattaatg cacaacattt
      181 aacctacatt cttccttatc ggatcctcaa aacccttaaa aacatatgcc tcaccctaac
      241 atattttcca attaaccctc aatatttctc tgtcacccgg cctctatttt ccattttctt
      301 ctttacccgc cacgcgtttt tttctttcaa atttttttct tccttcttct ttttcttcca
      361 cgtcctcttg cataaataaa taaaccgttt tgaaaccaaa ctcgcctctc tctctccttt
      421 ttgaaatatt tttgggtttg tttgatcctt tccttcccaa tctctcttgt ttaatatata
      481 ttcatttata tcacgctctc tttttatctt cctttttttc ctctctcttg tattcttcct
      541 tcccctttct actcaaacca agaagaaaaa gaaaaggtca atctttgtta aagaatagga
      601 tcttctacta catcagcttt tagatttttc acgcttactg cttttttctt cccaagatcg
      661 aaaatttact gaattaacaa ctagtggatc ccccgggctg caggaattca tgaaagcgtt
      721 aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat
      781 gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga
      841 agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg
      901 cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctg
//
