LOCUS       sequence-111497-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pcDNA 3.1 (+) FLAG-Ago2 S387E
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcacca tggactacaa
      121 agacgatgac gacaagggat ccgaattcgg tgttctctct gccattcccg cacttgcacc
      181 tcctgcgccg ccgcccccca tccaaggata tgccttcaag cctccaccta gacccgactt
      241 tgggacctcc gggagaacaa tcaaattaca ggccaatttc ttcgaaatgg acatccccaa
      301 aattgacatc tatcattatg aattggatat caagccagag aagtgcccga ggagagttaa
      361 cagggaaatc gtggaacaca tggtccagca ctttaaaaca cagatctttg gggatcggaa
      421 gcccgtgttt gacggcagga agaatctata cacagccatg ccccttccga ttgggaggga
      481 caaggtggag ctggaggtca cgctgccagg agaaggcaag gatcgcatct tcaaggtgtc
      541 catcaagtgg gtgtcctgcg tgagcttgca ggcgttacac gatgcacttt cagggcggct
      601 gcccagcgtc ccttttgaga cgatccaggc cctggacgtg gtcatgaggc acttgccatc
      661 catgaggtac acccccgtgg gccgctcctt cttcaccgcg tccgaaggct gctctaaccc
      721 tcttggcggg ggccgagaag tgtggtttgg cttccatcag tccgtccggc cttctctctg
      781 gaaaatgatg ctgaatattg atgtgtcagc aacagcgttt tacaaggcac agccagtaat
      841 cgagtttgtt tgtgaagttt tggattttaa aagtattgaa gaacaacaaa aacctctgac
      901 agattcccaa agggtaaagt ttaccaaaga aattaaaggt ctaaaggtgg agataacgca
      961 ctgtgggcag atgaagagga agtaccgtgt ctgcaatgtg acccggcggc cc
//
