LOCUS       sequence-111498-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes one a1 operator site upstream of minimal pCYC1 promoter 
            driving yeast enhanced BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: 1x_a1gRNAOp_pCYC1m_yeBFP2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     16..32
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
       61 cgggtactgt atgtacatac agtaggatcc tatggcatgc atgtgctctg tatgtatata
      121 aaactcttgt tttcttcttt tctctaaata ttctttcctt atacattagg acctttgcag
      181 cataaattac tatacttcta tagacacaca aacacaaata cacacactaa tctagataaa
      241 aaatggtttc caagggtgaa gaattattca ccggtgttgt ccctattttg gtcgaattgg
      301 acggtgatgt caacggtcac aagttctccg ttagaggtga aggtgaaggt gacgctacca
      361 acggtaagtt gaccttgaag ttcatctgta ccactggtaa gttgccagtt ccttggccaa
      421 ctttggttac cactttgtct cacggtgttc aatgtttcgc ccgttaccca gatcacatga
      481 agcaacatga tttctttaaa tccgccatgc cagaaggtta cgttcaagaa agaactatct
      541 tcttcaagga cgatggtact tacaaaacta gagccgaagt caagttcgaa ggtgatactt
      601 tagtcaatag aatcgaattg aagggtgttg atttcaagga agatggtaac attttgggtc
      661 acaagttaga atacaatttc aactcccata atatttacat tatggctgtt aagcaaaaga
      721 acggtattaa ggtcaacttt aaaatcagac acaacgttga agacggttct gtccaattag
      781 ctgaccacta ccaacaaaac actccaattg gtgacggtcc agtcttatta ccagattctc
      841 attacttgtc tacccaatct gtcttgtcta aggacccaaa
//
