LOCUS       sequence-111499-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  controls the expression of S. pyogenes dCas9 from a Tc‐inducible 
            PTet promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PTet-dCas9
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             154..165
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    213..231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(398..417)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(482..501)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ccctataaga ttttttttga tactgtggcg gtctgtattt cccagaacct tgaacttttt
       61 agacggaacc ttatattcat cagtgatcac cgcccatccg acgctatttg tgccgatagc
      121 taagcctatt gagtatttct tatccataga tcctttctcc tctttagatc ttttgaagtg
      181 ctcattatct ctatcactga tagggatgtc aatctctatc actgataggg aaaaaagcca
      241 acggtggagt aaagcgggag accagaaaca aaaaaaggcc ccccgttagg gaggccttca
      301 ataattggtt gtgtctcaaa atctctgatg tacattgcac aagataaaaa tatatcatca
      361 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      421 caacgggaaa cgtcttgctc caggccgcga ttaaattcca acatggatgc tgatttatat
      481 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
      541 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
      601 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
      661 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat cc
//
