LOCUS       sequence-111501-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pcDNA 3.1 (+) FLAG-Ago2 S387E
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actcgagcgg ccgctcaagc aaagtacatg gtgcgcagag tgtcttggtg aacctggacc
       61 gccttggcca gtgcttggtg gtctcgcccg ttactctgcc cagaggtatg gcttccttca
      121 gcactgtcat gttccttatc caccaggtgg tacctggccc ggaaggccac caggtgagcg
      181 tagtatgctg gcgctgggat ggacacggag cgtgtgcagc gcacgtaggt gtgacacagc
      241 tggtaggtta ggatctgcag ctcatcagag gagaaacgat tgtcgtccca gaggacgtga
      301 tagtgcgaag gcctgcttgt cccctggatg ccagcgtgac tacacaggta gaagtcgaac
      361 tcggtggggt gggtgatttt cgtgtccaca gtcgtgcctg ctggaatgtt tccacttttc
      421 ccaacccgct cgttcttgtc agtgcagaag agccgggtgt ggtgcctctt ctgcaccacg
      481 atgaaggtga tcccgggctg gtagtctttt tctagcttga tacaggcctc acggatggcc
      541 agcaactcgt ggtggagaac ctgctggaac tggccttcag agacaccgtc gcggtagaag
      601 atgatgcggg tgggcttgaa gcgcgtggac ttgtagaact ggatgaggag ctcgcggacc
      661 atggcggcca ggtcttgtat gatctcctgc cggtgctgct gcacgcgcac ggtggcgcag
      721 tagcgattgg ggtgggcgtc catgctgccc accacggcgg caatggaggg cttcttccca
      781 tccccggcgg gggggtgagt gacgtctgct cccagaaaga tgacgggctg ctggaacacc
      841 ggcggcctgc cctggggcag caggatgttg ttcacgcctc ccagcttgac gttgatcttc
      901 aggcagaggt tggacagggt ctgtggcgtg gtcctctgca cgttcttcat ctgcacgcac
      961 tgcgtgg
//
