LOCUS       sequence-111502-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c5 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_c5gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(350..412)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(425..500)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agtggaacaa aagcaaatga aggatttcat gcgtttgtac tctaatctgg tagaaagatg
       61 tttcacagac tgtgtcaatg acttcacaac atcaaagcta accaataagg aacaaacatg
      121 catcatgaag tgctcagaaa agttcttgaa gcatagcgaa cgtgtagggc agcgtttcca
      181 agaacaaaac gctgccttgg gacaaggctt gggccgataa ggtgtactgg cgtatatata
      241 tctaattatg tatctctggt gtagcccatt tttagcatgt aaatataaag agaaaccata
      301 tctaatctaa ccaaatccaa acaaaattca atagttacta tcgctttttt ctttctgtat
      361 cgcaaataag tgaaaattaa aaaagaaaga ttaaattgga agttggatat ggctcgagaa
      421 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
      481 gctatttcta gctctaaaac ttgcagcata aattactata aagcttctgc caatcgcagc
      541 tcccagagtt tcgttcccag ccgcgagcac cacagcgtac catgtgcgcc acgaggcctc
      601 aaacgtaaaa caatcgaaac gaaaagaaac agactatagg ggagtataga gacagccggc
      661 caataagaag aggaaaaaga aatactagcg tttatcaatg tgggtcgtta acatatcctg
      721 ttgacaatga ttacaggtta aaaggtagcg taagtgaata ttaactatgg atattcttat
      781 acttagataa gagacatata aaacacacga tgattgattg
//
