LOCUS       sequence-111507-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLM
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        130..317
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             318..980
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 aattccgttt taagagcttg gtgagcgcta ggagtcactg ccaggtatcg tttgaacacg
       61 gcattagtca gggaagtcat aacacagtcc tttcccgcaa ttttcttttt ctattactct
      121 tggcctcctc tagtacactc tatatttttt tatgcctcgg taatgatttt catttttttt
      181 tttccaccta gcggatgact cttttttttt cttagcgatt ggcattatca cataatgaat
      241 tatacattat ataaagtaat gtgatttctt cgaagaatat actaaaaaat gagcaggcaa
      301 gataaacgaa ggcaaagatg acagagcaga aagccctagt aaagcgtatt acaaatgaaa
      361 ccaagattca gattgcgatc tctttaaagg gtggtcccct agcgatagag cactcgatct
      421 tcccagaaaa agaggcagaa gcagtagcag aacaggccac acaatcgcaa gtgattaacg
      481 tccacacagg tatagggttt ctggaccata tgatacatgc tctggccaag cattccggct
      541 ggtcgctaat cgttgagtgc attggtgact tacacataga cgaccatcac accactgaag
      601 actgcgggat tgctctcggt caagctttta aagaggccct aggggccgtg cgtggagtaa
      661 aaaggtttgg atcaggattt gcgcctttgg atgaggcact ttccagagcg gtggtagatc
      721 tttcgaacag gccgtacgca gttgtcgaac ttggtttgca aagggagaaa gtaggagatc
      781 tctcttgcga gatgatcccg cattttcttg aaagctttgc agaggctagc agaattaccc
      841 tccacgttga ttgtctgcga ggcaagaatg atcatcaccg tagtgagagt gcgttcaagg
      901 ctcttgcggt tgccataaga gaagccacct cgcccaatgg taccaacgat gttccctcca
      961 ccaaaggtgt tcttatgtag tgacaccgat tatttaaagc tgcagcatac gatatatata
     1021 catg
//
