LOCUS       sequence-111508-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c1 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_c1gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(347..409)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(422..497)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggaacaaaag caaatgaagg atttcatgcg tttgtactct aatctggtag aaagatgttt
       61 cacagactgt gtcaatgact tcacaacatc aaagctaacc aataaggaac aaacatgcat
      121 catgaagtgc tcagaaaagt tcttgaagca tagcgaacgt gtagggcagc gtttccaaga
      181 acaaaacgct gccttgggac aaggcttggg ccgataaggt gtactggcgt atatatatct
      241 aattatgtat ctctggtgta gcccattttt agcatgtaaa tataaagaga aaccatatct
      301 aatctaacca aatccaaaca aaattcaata gttactatcg cttttttctt tctgtatcgc
      361 aaataagtga aaattaaaaa agaaagatta aattggaagt tggatatggc tcgagaaaaa
      421 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacgtgct
      481 atttctagct ctaaaactta gacattttaa tatctagaag cttctgccaa tcgcagctcc
      541 cagagtttcg ttcccagccg cgagcaccac agcgtaccat gtgcgccacg aggcctcaaa
      601 cgtaaaacaa tcgaaacgaa aagaaacaga ctatagggga gtatagagac agccggccaa
      661 taagaagagg aaaaagaaat actagcgttt atcaatgtgg gtcgttaaca tatcctgttg
      721 acaatgatta caggttaaaa ggtagcgtaa gtgaatatta actatggata ttcttatact
      781 tagataagag acatataaaa cacacgatga ttgattgatt tttatgacca atatatgtaa
      841 ttcgta
//
