LOCUS       sequence-111509-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c7 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_c7gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(348..410)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(423..498)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tggaacaaaa gcaaatgaag gatttcatgc gtttgtactc taatctggta gaaagatgtt
       61 tcacagactg tgtcaatgac ttcacaacat caaagctaac caataaggaa caaacatgca
      121 tcatgaagtg ctcagaaaag ttcttgaagc atagcgaacg tgtagggcag cgtttccaag
      181 aacaaaacgc tgccttggga caaggcttgg gccgataagg tgtactggcg tatatatatc
      241 taattatgta tctctggtgt agcccatttt tagcatgtaa atataaagag aaaccatatc
      301 taatctaacc aaatccaaac aaaattcaat agttactatc gcttttttct ttctgtatcg
      361 caaataagtg aaaattaaaa aagaaagatt aaattggaag ttggatatgg ctcgagaaaa
      421 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
      481 tatttctagc tctaaaacat ggcatgcatg tgctctgtaa gcttctgcca atcgcagctc
      541 ccagagtttc gttcccagcc gcgagcacca cagcgtacca tgtgcgccac gaggcctcaa
      601 acgtaaaaca atcgaaacga aaagaaacag actatagggg agtatagaga cagccggcca
      661 ataagaagag gaaaaagaaa tactagcgtt tatcaatgtg ggtcgttaac atatcctgtt
      721 gacaatgatt acaggttaaa aggtagcgta agtgaatatt aactatggat attcttatac
      781 ttagataaga gacatataaa acacacgatg attgattgat ttttatgacc aatatatgta
      841 attc
//
