LOCUS       sequence-111513-         537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a1 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_a1gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(286..348)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(361..436)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acagactgtg tcaatgactt cacaacatca aagctaacca ataaggaaca aacatgcatc
       61 atgaagtgct cagaaaagtt cttgaagcat agcgaacgtg tagggcagcg tttccaagaa
      121 caaaacgctg ccttgggaca aggcttgggc cgataaggtg tactggcgta tatatatcta
      181 attatgtatc tctggtgtag cccattttta gcatgtaaat ataaagagaa accatatcta
      241 atctaaccaa atccaaacaa aattcaatag ttactatcgc ttttttcttt ctgtatcgca
      301 aataagtgaa aattaaaaaa gaaagattaa attggaagtt ggatatggct cgagaaaaaa
      361 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
      421 tttctagctc taaaacactg tatgtacata cagtaaaagc ttctgccaat cgcagctccc
      481 agagtttcgt tcccagccgc gagcaccaca gcgtaccatg tgcgccacga ggcctca
//
