LOCUS       sequence-111525-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-Ago2 S387E mutant lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pHAGE GFP-Ago2 S387E
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      680..689
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttaggatcta tcgatttaag caaagtacat ggtgcgcaga
       61 gtgtcttggt gaacctggac cgccttggcc agtgcttggt ggtctcgccc gttactctgc
      121 ccagaggtat ggcttccttc agcactgtca tgttccttat ccaccaggtg gtacctggcc
      181 cggaaggcca ccaggtgagc gtagtatgct ggcgctggga tggacacgga gcgtgtgcag
      241 cgcacgtagg tgtgacacag ctggtaggtt aggatctgca gctcatcaga ggagaaacga
      301 ttgtcgtccc agaggacgtg atagtgcgaa ggcctgcttg tcccctggat gccagcgtga
      361 ctacacaggt agaagtcgaa ctcggtgggg tgggtgattt tcgtgtccac agtcgtgcct
      421 gctggaatgt ttccactttt cccaacccgc tcgttcttgt cagtgcagaa gagccgggtg
      481 tggtgcctct tctgcaccac gatgaaggtg atcccgggct ggtagtcttt ttctagcttg
      541 atacaggcct cacggatggc cagcaactcg tggtggagaa cctgctggaa ctggccttca
      601 gagacaccgt cgcggtagaa gatgatgcgg gtgggcttga agcgcgtgga cttgtagaac
      661 tggatgagga gctcgcggac catggcggcc aggtcttgta tgatctcctg ccggtgctgc
      721 tgcacgcgca cggtggcgca gtagcgattg gggtgggcgt ccatgctgcc caccacggcg
      781 gcaatggagg gcttcttccc atccccggcg ggggggtgag tgacgtctgc tcccagaaag
      841 atgacgggct gctggaacac cggcggcctg ccctggggca gcaggatgtt gttcacgcct
      901 cccagcttga cgttgatctt caggcagagg ttggacaggg tctgtggcgt ggtcctctgc
      961 acgttcttca tctgcacgca ctg
//
