LOCUS       sequence-111526-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes yeast-optimized dCas9 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pTPGI_dCas9
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     84..107
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             153..173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatatt acttcttatt caaatgtaat
       61 aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaacc
      121 ccggatccac gattaaaaga atgtctagag ccccaaagaa gaagagaaaa gttagacccg
      181 gggataagaa atactctatt ggtttggcta tcggtacaaa ctctgttggt tgggctgtta
      241 ttactgatga atacaaggtt ccatccaaga agttcaaggt tttgggtaac actgatagac
      301 actccatcaa aaagaacttg attggtgcct tgttgttcga ttctggtgaa actgctgaag
      361 ctactagatt gaaaagaacc gctagaagaa gatacaccag aagaaagaac agaatctgct
      421 acttgcaaga aatcttctcc aacgaaatgg ccaaggttga tgattcattc ttccacagat
      481 tggaagaatc cttcttggtc gaagaagata agaagcacga aagacatcca atcttcggta
      541 acatcgttga tgaagttgct taccacgaaa agtacccaac tatctaccat ttgagaaaga
      601 agttggttga ctctaccgat aaggctgatt tgagattgat ctatttggct ttggcccaca
      661 tgattaagtt cagaggtcat ttcttgatcg aaggtgattt gaacccagat aactccgatg
      721 ttgataagtt gttcatccaa ttagtccaaa cctacaatca attattcgaa gaaaacccaa
      781 tcaacgcctc tggtgttgat gctaaagcta ttttgtctgc caga
//
