LOCUS       sequence-111532-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-Ago2 S387A mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pGEX 4T-1 GST-Ago2 S387A
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcgcgaggc agatcgtcag tcagtcacga tgcggccgct caagcaaagt acatggtgcg
       61 cagagtgtct tggtgaacct ggaccgcctt ggccagtgct tggtggtctc gcccgttact
      121 ctgcccagag gtatggcttc cttcagcact gtcatgttcc ttatccacca ggtggtacct
      181 ggcccggaag gccaccaggt gagcgtagta tgctggcgct gggatggaca cggagcgtgt
      241 gcagcgcacg taggtgtgac acagctggta ggttaggatc tgcagctcat cagaggagaa
      301 acgattgtcg tcccagagga cgtgatagtg cgaaggcctg cttgtcccct ggatgccagc
      361 gtgactacac aggtagaagt cgaactctgt ggggtgggtg attttcgtgt ccacagtcgt
      421 gcctgctgga atgtttccac ttttcccaac ccgctcgttc ttgtcagtgc agaagagccg
      481 ggtgtggtgc ctcttctgca ccacgatgaa ggtgatccct ggctggtagt ctttttctag
      541 cttgatacag gcctcacgga tggccagcaa ctcgtggtgg agaacctgct ggaactggcc
      601 ttcagagaca ccgtcgcggt agaagatgat gcgggtgggc ttgaagcgcg tggacttgta
      661 gaactggatg aggagctcgc ggaccatggc ggccaggtct tgtatgatct cctgccggtg
      721 ctgctgcaca cgcacggtgg cgcagtagcg attggggtgg gcgtccatgc tgcccaccac
      781 ggcggcaatg gagggcttct tcccatcccc ggcggggggg tgagtgacgt ctgctcccag
      841 aaagatgacg ggctgctgga acaccggcgg cctgccctgg ggcagc
//
