LOCUS       sequence-111535-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker gene LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pK
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..40)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        154..393
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
       61 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcggcgcg
      121 tcgaggaaaa ctcttcaata gttttgccag cggaattcca cttgcaatta cataaaaaat
      181 tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa catgaacatc
      241 gcccaaatgt atttgaagac ccgctgggag aagttcaaga tatataagta acaagcagcc
      301 aatagtataa aaaaaaatct gagtttatta cctttcctgg aatttcagtg aaaaactgct
      361 aattatagag agatatcaca gagttactca ctaatgacta acgaaaaggt ctggatagag
      421 aagttggata atccaactct ttcagtgtta ccacatgact ttttacgccc acaacaagaa
      481 ccttatacga aacaagctac atattcgtta cagctacctc agctcgatgt gcctcatgat
      541 agtttttcta acaaatacgc tgtcgctttg agtgtatggg ctgcattgat atatagagta
      601 accggtgacg atgatattgt tctttatatt gcgaataaca aaatcttaag attcaatatt
      661 caaccaacgt ggtcatttaa tgagctgtat tctacaatta acaatgagtt gaacaagctc
      721 aattctattg aggccaattt ttcctttgac gagctagctg aaaaaattca aagttgccaa
      781 gatctggaaa ggacccctca gttgttccgt ttggcctttt tggaaaacca agatttcaaa
      841 ttagacgagt tcaagcatca tttagtggac tttgctttga atttggatac cagtaataat
      901 gcgcatgttt tgaacttaat ttataacagc ttactgtatt cgaatgaaag agtaaccatt
      961 gt
//
