LOCUS       sequence-111545-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-M13::GAL4DBDo
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..119
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(123..131,132..146)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             174..230
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             273..713
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     intron          812..877
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tagggaattg ggaattcgtt aacagatcgg gggatcaatt cgttaacaga tccaccatgg
       61 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      121 gggatctgta cgacgatgac gataaggatc tcgccaccat ggtcgactca tcacgtcgta
      181 agtggaataa gacaggtcac gcagtcagag ctataggtcg gctgagctca tctagcggag
      241 gaggtggtgg aggtggaggt ggaggtacta gtatgaagct gctgagtagt attgaacaag
      301 cgtgtgacat atgccgactt aagaagctga agtgcagcaa ggaaaagccg aagtgcgcca
      361 aatgtcttaa gaacaattgg gagtgccggt actcgccaaa gacgaagcga agccctttga
      421 cacgcgctca tttgactgaa gtggagagtc gcctggagcg ccttgagcag ttgttcctcc
      481 tcatttttcc ccgtgaagac ctcgacatga tactgaagat ggatagtctc caagatatta
      541 aggccctgct cactggcctg tttgttcagg ataacgtcaa caaggatgcc gtgacggacc
      601 gccttgcctc ggtggagaca gatatgccac ttaccttgcg gcagcatcgc atctcggcta
      661 ccagcagtag tgaagaaagt tccaataagg gtcaacggca actgacggta tcgtaactcg
      721 agggtacctc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
      781 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
      841 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
      901 atgg
//
