LOCUS       sequence-111548-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-frt-stop-frt-p65AD::CaM
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..120
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          240..305
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             435..455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(764..783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tagggaattg ggaattcgtt aacagatcgg gggatcaatt cgttaacaga tctgagggta
       61 cccggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
      121 agagcgcttt tgaagctaga gaattcgcgc actagtgcta gaggatcttt gtgaaggaac
      181 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
      241 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
      301 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
      361 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
      421 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
      481 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
      541 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
      601 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
      661 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      721 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      781 ttcacaaata aagcattt
//
