LOCUS       sequence-111554-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pUASTattB-MKII::nlsLexADBDo
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    134..167
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    200..294
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        313..551
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     498..519
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
       61 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
      121 gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct agcggatcca
      181 agcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      241 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaga
      301 ctctagcgag cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
      361 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
      421 acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
      481 aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga
      541 agagaactct gaatagggaa ttgggaattc gttaacagat cgggggatca attcgttaac
      601 agatctcacc atgtttaatg cgcggcgcaa gctaaaggga gccatactta cgacaatgtt
      661 ggcgaccaga aatttttcgg ctagcggagg aggtggtgga ggtggaggtg gaggtactag
      721 tccacccaag aagaagcgaa
//
