LOCUS       sequence-111559-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pUASTattB-p65AD::CaM
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..171
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    204..298
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        317..555
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     502..523
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             645..665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
       61 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
      121 actggaacta ggctagcata acttcgtata atgtatgcta tacgaagtta tgctagcgga
      181 tccaagcttg catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
      241 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      301 gagactctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
      361 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
      421 ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac
      481 cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac
      541 aagaagagaa ctctgaatag ggaattggga attcgttaac agatcggggg atcaattcgt
      601 taacagatct caacatggat aaagcggaat taattcccga gcctccaaaa aagaagagaa
      661 aggtcgaatt gggatccacg ccgatggagt tccagtacct gcccgatacg gatgaccgtc
      721 accgtatcga agaaaagcgg aagcgaacct atgaaacctt caagtccatc atgaaa
//
