LOCUS       sequence-111581-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-tagged biotinylation enzyme for co-expression with 
            bio-proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: secreted BirA-Flag
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgaattccc tctagagccg ccaccatggg cagacacctc ctgctgctcc tgttcagcat
       61 cctgtacatg ctgctggccc aggccaagga caacaccgtg cccctgaagc tgatcgccct
      121 gctggccaac ggcgagttcc acagcggcga gcagctgggc gagaccctgg gcatgagcag
      181 agccgctatc aacaagcaca tccagaccct gagagactgg ggcgtggacg tgttcaccgt
      241 gcccggcaag ggctacagcc tgcccgagcc catccagctg ctgaacgcca agcagatcct
      301 gggccagctg gacggcggaa gcgtggccgt gctgcccgtg atcgacagca ccaaccagta
      361 cctgctggac agaatcggcg agctgaagag cggcgacgcc tgcatcgccg agtaccagca
      421 ggccggcaga ggcagaagag gcagaaagtg gttcagcccc tttggcgcca acctgtacct
      481 gagcatgttc tggagactgg agcagggccc cgcagccgca atcggcctga gcctggtgat
      541 cggcatcgtg atggccgagg tgctgagaaa gctgggcgcc gacaaggtga gagtgaagtg
      601 gcccaacgac ctgtacctgc aggacagaaa gctggccggc atcctggtgg agctgaccgg
      661 caagaccggc gacgccgcac agatcgtgat cggcgccggc atcaacatgg ccatgagaag
      721 agtggaggaa agcgtggtga accagggctg gatcaccctg caggaggccg gcatcaacct
      781 ggacagaaac accctggcag ccatgctgat cagagagctg agagccgcac tggagctgtt
      841 cgagca
//
