LOCUS       sequence-111590-       10726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cloning vector for human POLQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pBluescript II SK--POLQ
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8538..8558)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8538..8556)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8577..8593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8577..8593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8590..8612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8601..8617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8625..8631,8632..8649,8650..8655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8670..8691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8808..8825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8979..9567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9059..9078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9738..10529,10530..10598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10361..10380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10599..10703)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atcctagcac catgaatctt ctgcgtcgga gtgggaaacg gcggcgttca gaatcaggct
      781 cagattcgtt ctcgggaagc ggcggtgaca gcagtgccag cccccagttc ctctccgggt
      841 ccgtgctgag cccgccgccc ggccttggtc gctgcctgaa ggccgcagct gcaggagaat
      901 gcaagcctac agttcctgac tacgaaatag acaagctact attggcaaac tggggacttc
      961 ctaaagcagt tctggaaaaa taccacagtt ttggtgtaaa aaagatgttt gaatggcagg
     1021 cagagtgcct tttgcttgga caagtcctgg aaggaaagaa tttagtttat tcagctccta
     1081 caagtgctgg gaagactctt gtggcagaat tacttatttt gaagcgggtt ttggaaatgc
     1141 ggaagaaagc tttgtttatt cttccctttg tttctgtggc taaagagaag aaatactacc
     1201 tccagagtct gtttcaggaa gtaggaataa aagtagacgg ttatatgggc agcacctctc
     1261 catcaaggca tttctcttca ttggatattg cagtctgcac aattgagaga gccaatggtc
     1321 tgatcaatcg cctcatagag gaaaataaga tggatctgtt aggaatggtg gttgtggatg
     1381 aattacatat gctgggagac tctcaccgag ggtatctgct ggaacttttg ctgaccaaga
     1441 tttgctatat tactcggaaa tcagcatctt gtcaggcaga tctagccagt tctctgtcta
     1501 atgctgtgca aatcgttggc atgagtgcta cccttcctaa tttggagctt gtggcttcct
     1561 ggttgaatgc tgaactctac cataccgact ttcgccctgt accgcttttg gagtcagtaa
     1621 aagttggaaa ttccatatat gactcttcaa tgaaacttgt gagggaattt gagcccatgc
     1681 ttcaagtgaa gggagatgag gaccatgttg ttagcttatg ttatgagacg atttgtgata
     1741 accattcagt attacttttt tgtccatcaa agaaatggtg tgagaagctg gcagatatca
     1801 ttgctcgaga gttttataat ctacatcatc aagctgaggg tttggtgaaa ccctctgaat
     1861 gcccaccagt aattctggaa caaaaagaac tcctggaagt gatggatcag ttaagacgtt
     1921 tgccttcagg actggactct gtattacaga aaactgtacc atggggagta gcatttcatc
     1981 atgcaggtct tacttttgag gagagggata tcattgaagg agcctttcgt caaggtctca
     2041 ttcgggtctt ggcggcaact tctactcttt cttctggggt gaatttacct gcacgtcgtg
     2101 tgattattcg aacccctatt tttggtggtc gacctctaga tattcttact tataagcaga
     2161 tggttggtcg tgctggcagg aaaggagtgg acacagtagg cgagagtatc ttaatttgta
     2221 agaactctga gaaatcaaaa ggcatagctc tccttcaggg ttctctaaag cctgttcgca
     2281 gctgtctgca aagacgagaa ggagaagaag taactggcag catgatacga gctattctgg
     2341 agataatagt tggtggagtg gcaagtacat cacaagatat gcatacttat gctgcctgca
     2401 catttttggc tgcaagtatg aaagaaggga agcaaggaat tcagagaaat caagagtctg
     2461 ttcagcttgg agcgattgag gcctgtgtga tgtggctact agaaaatgaa ttcatccaga
     2521 gtacagaagc cagtgatgga acagaaggaa aggtgtatca tccaacacat cttggttcgg
     2581 ccactctttc ttcttcactt tctccagctg atactttaga tatttttgct gacctgcaaa
     2641 gagcaatgaa gggctttgtt ttagagaatg atcttcatat tctctatctg gttacaccta
     2701 tgtttgagga ttggactact attgattggt atcgattttt ctgtttatgg gagaagttgc
     2761 caacttcaat gaaaagggtg gcagagctag tgggagttga agaggggttc ttggcccgtt
     2821 gtgtgaaagg aaaagtagta gccagaactg agagacagca tcgacaaatg gccatccata
     2881 aaaggttttt caccagtctt gtgctattag atttaatcag tgaagttccc ttaagggaaa
     2941 taaatcagaa atatggatgc aatcgtgggc agattcaatc tttgcaacag tcagctgctg
     3001 tttatgcagg gatgattaca gtattttcca accgtctggg ctggcacaac atggaactac
     3061 tactttccca atttcagaag cgtcttacgt ttggcatcca gagggagctg tgtgacctgg
     3121 ttcgggtatc cttactaaat gctcagagag ccagggttct ctatgcttct ggctttcata
     3181 ctgtggcaga ccttgctaga gcaaatattg tggaggtgga ggtgattctg aaaaatgctg
     3241 tgcctttcaa aagtgcccgg aaggcagtgg atgaggaaga ggaagcagtt gaagaacgtc
     3301 gcaatatgcg aactatctgg gtgactggca gaaaaggttt aactgaaagg gaagcagcag
     3361 cccttatagt ggaagaagcc agaatgattc tgcagcagga cttagttgaa atgggagtgc
     3421 aatggaatcc atgtgccctg ttacattcta gtacatgctc attgactcat agtgagtccg
     3481 aagtaaagga acacacattt atatcccaaa ctaagagttc ttataaaaaa ttaacatcaa
     3541 agaacaaaag taacacaata tttagtgatt cttatattaa gcattcacca aatatagtgc
     3601 aagacttaaa taaaagtaga gagcatacaa gttcctttaa ttgtaatttc cagaatggga
     3661 atcaagaaca tcagagatgt tccattttca gagcaagaaa acgggcctct ttagatataa
     3721 ataaagagaa gccaggagcc tctcagaatg aggggaaaac aagtgataag aaagttgttc
     3781 agactttttc acagaaaaca aaaaaggcac ctttgaattt caattcagaa aagatgagca
     3841 gaagttttcg atcttggaaa cgtagaaagc atctaaagcg atctagggac agcagccccc
     3901 tgaaagactc tggagcgtgt agaatccatt tacaaggaca gactctgtct aatcctagtc
     3961 tttgtgaaga cccgtttacc ttagatgaga agaaaacgga atttagaaat tcagggccat
     4021 ttgctaaaaa tgtatctttg agtggtaagg aaaaagataa taaaacatca ttcccattac
     4081 aaataaagca aaattgttca tggaacataa cactaactaa tgataatttt gtggagcata
     4141 ttgtcacagg atctcagagt aaaaatgtga cttgtcaggc cactagtgtg gttagtgaaa
     4201 agggcagagg agtagctgtt gaggcagaaa aaataaatga agtgctgata caaaatggtt
     4261 caaaaaacca gaatgtttat atgaaacacc atgacatcca tccaattaac cagtacctgc
     4321 gaaagcaatc tcatgaacag acaagcacta ttaccaaaca gaaaaatata atagagagac
     4381 aaatgccctg tgaagcagtc agtagttaca taaatagaga ctcaaatgtt actatcaatt
     4441 gtgaaaggat aaagcttaat acagaggaaa ataaaccaag tcattttcag gcattaggag
     4501 atgatataag cagaactgtg atacccagtg aagtacttcc atcagctgga gcatttagca
     4561 aatcagaagg ccagcatgag aattttctaa atatttctag actacaagaa aaaacaggta
     4621 cttatacaac aaacaaaact aaaaataatc atgtttctga cttaggttta gtcctctgtg
     4681 attttgaaga tagtttctat ctggatactc agtcagagaa aataatacaa cagatggcaa
     4741 ctgaaaatgc caaactagga gcaaaggaca ccaacctggc agcagggata atgcagaaga
     4801 gcttagtcca acagaactca atgaactctt ttcagaagga gtgtcacatt ccttttcctg
     4861 ctgaacagca ccctctagga gcgactaaga tagatcattt ggaccttaag actgtaggta
     4921 ctatgaaaca aagcagtgat tcacatgggg ttgatatcct gactccagaa agcccgattt
     4981 tccattctcc aatactattg gaggaaaatg gtcttttttt aaaaaagaat gaagtttctg
     5041 ttactgattc acaattaaat agttttcttc aaggttatca aacacaagaa actgtgaaac
     5101 cagttatact tctgattcct caaaagagaa ctcccactgg tgtagaagga gaatgtcttc
     5161 cagttcctga aacaagtttg aatatgagtg atagtttact atttgatagc ttcagtgatg
     5221 actatctagt aaaagaacaa ttacctgata tgcaaatgaa agaacccctt ccttcagaag
     5281 taacatcaaa ccattttagt gattctctgt gtctacaaga agacctaatt aaaaaatcaa
     5341 atgtaaatga gaatcaagat acccaccagc agttgacttg ttccaatgat gaatctatta
     5401 tattttcaga aatggattct gttcagatgg ttgaagcttt ggacaatgtg gatatatttc
     5461 ctgtccaaga gaagaatcat actgtagtat ctcctagagc attagaacta agtgatccag
     5521 tacttgatga gcaccaccaa ggtgatcaag atggaggaga tcaagatgaa agggctgaaa
     5581 aatcaaaatt aactgggacc aggcaaaatc attcattcat atggtcaggg gcatcatttg
     5641 atctaagtcc aggactgcaa aggattttag ataaagtatc cagtcctcta gaaaatgaaa
     5701 agctaaaatc aatgactata aacttttcca gtttgaatag aaaaaataca gagttaaatg
     5761 aagaacaaga agttatttca aacttggaga caaaacaagt gcagggaatt tcattttctt
     5821 ctaataatga agtaaaaagc aagattgaga tgctagaaaa caatgccaat catgatgaaa
     5881 cctcatccct cttacctcgt aaagaaagta atatagttga tgataatggt ctcattcctc
     5941 ctacacccat tccaacatct gcttctaagc tgacatttcc agggattctt gaaacacctg
     6001 taaacccttg gaaaactaat aatgttttac aacctggtga aagttattta tttggctcac
     6061 cttcagatat taaaaaccac gatttaagtc cagggagtag aaatgggttc aaagacaaca
     6121 gccctattag tgacacaagc ttttcacttc agttatcaca ggatggatta cagttaactc
     6181 cagcctcaag cagttcagaa agtttgtcca taattgatgt agcaagtgac caaaatcttt
     6241 tccaaacatt cattaaggag tggcggtgca aaaagcgatt ttccatctca ctggcttgtg
     6301 aaaagattag aagtttgaca tcttctaaaa ctgctactat tggcagtagg tttaagcaag
     6361 ctagctcacc tcaggaaatt cctattagag atgatggatt tcccattaaa ggttgtgatg
     6421 acaccttggt ggttggactg gcagtatgct ggggtggaag ggatgcctat tatttttcac
     6481 tgcagaagga acaaaagcat tctgaaatta gtgccagttt ggttccacct tctttagatc
     6541 caagcctgac tttgaaagac aggatgtggt accttcaatc ttgcttgcga aaggaatctg
     6601 ataaagaatg ttctgttgtc atctatgact tcatccagag ctataaaatt cttcttcttt
     6661 cttgtggcat ctccttggag caaagttatg aagatcctaa ggtggcatgc tggttactag
     6721 atccagattc tcaggagccg actcttcata gcatagttac cagttttctt cctcatgagc
     6781 ttccactcct agaagggatg gagaccagcc aagggattca aagcctgggg ctaaatgctg
     6841 gcagtgagca ttctgggcga tacagagcat ctgtggagtc cattctcatc ttcaactcta
     6901 tgaatcagct caactctttg ttgcagaagg aaaaccttca agatgttttc cgtaaggtgg
     6961 aaatgccctc tcagtactgc ttggccttgc tagaactaaa tggaattggc tttagtactg
     7021 cagaatgtga aagtcagaaa catataatgc aagccaagct ggatgcaatt gagacccagg
     7081 cctatcaact agctggccac agtttttctt tcaccagttc agatgacatc gctgaggttt
     7141 tatttttgga attgaagttg cccccaaata gagagatgaa aaaccaaggc agcaagaaaa
     7201 ctctgggttc taccagaaga gggattgaca atggacgcaa gctaaggctg ggaagacagt
     7261 tcagcactag taaggacgtt ttaaataaat taaaggcatt acatccttta ccaggcttga
     7321 tattagaatg gagaagaatc actaatgcta ttaccaaagt ggtctttccc cttcagcggg
     7381 aaaagtgtct taatcctttt cttggaatgg aaagaatcta tcctgtatca cagtcgcaca
     7441 ctgctacagg acgaataacc tttacagaac caaatattca gaatgtgcca agagattttg
     7501 aaatcaaaat gccaacacta gtaggagaaa gcccaccttc tcaagctgta ggcaaaggcc
     7561 tacttcccat gggcagagga aaatataaga agggtttcag cgtgaatcct agatgccagg
     7621 cacagatgga ggagagagct gcagacagag gaatgccatt ttcaattagc atgcgacatg
     7681 cctttgtgcc tttcccaggt ggttcaatac tggctgctga ctactctcag cttgaactga
     7741 ggatcttggc tcatttatcc catgatcgtc gtctcattca agtgttaaac actggagctg
     7801 atgttttcag gagcattgca gcagagtgga agatgattga gccagagtct gttggggatg
     7861 atctgaggca gcaggcaaaa cagatttgct atgggatcat ttatggaatg ggagctaaat
     7921 ctttgggaga gcagatgggc attaaagaaa atgatgctgc atgctatatt gactccttca
     7981 aatccagata cacagggatt aatcaattca tgacagagac agtgaagaat tgtaaaagag
     8041 acggatttgt tcagaccatt ttgggaaggc gtagatattt gccaggaatc aaagacaaca
     8101 acccttatcg taaagctcac gctgagcgtc aagctatcaa cacaatagtc caaggatcag
     8161 cagctgatat tgtcaaaata gccacagtta acattcagaa gcaattagag accttccact
     8221 caaccttcaa atcccatggt catcgagagg gtatgctcca aagtgaccga acaggattgt
     8281 cacgaaagag aaaactgcaa gggatgttct gcccaatcag aggaggcttc ttcatccttc
     8341 aactccatga tgaactccta tatgaagtgg cagaagaaga tgttgttcag gtagctcaga
     8401 ttgtcaagaa tgaaatggaa agtgctgtaa aactgtctgt gaaattgaaa gtgaaagtga
     8461 aaataggcgc cagctgggga gagctaaagg actttgatgt ggcggccgcc accgcggtgg
     8521 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     8581 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8641 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8701 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8761 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8821 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8881 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8941 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9001 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9061 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9181 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9301 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9361 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9421 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9481 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9541 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9601 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9661 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9721 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9781 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9841 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9901 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9961 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10021 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10081 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10141 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10201 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10261 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10321 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10381 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10441 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10501 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10561 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10621 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10681 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
