LOCUS       sequence-111595-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-M13::GAL4DBDo
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..548)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             complement(591..647)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(675..689,690..698))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(702..734)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(738..755)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(855..876)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
       61 gaagtaaggt tccttcacaa agatcctcta gaggtaccct cgagttacga taccgtcagt
      121 tgccgttgac ccttattgga actttcttca ctactgctgg tagccgagat gcgatgctgc
      181 cgcaaggtaa gtggcatatc tgtctccacc gaggcaaggc ggtccgtcac ggcatccttg
      241 ttgacgttat cctgaacaaa caggccagtg agcagggcct taatatcttg gagactatcc
      301 atcttcagta tcatgtcgag gtcttcacgg ggaaaaatga ggaggaacaa ctgctcaagg
      361 cgctccaggc gactctccac ttcagtcaaa tgagcgcgtg tcaaagggct tcgcttcgtc
      421 tttggcgagt accggcactc ccaattgttc ttaagacatt tggcgcactt cggcttttcc
      481 ttgctgcact tcagcttctt aagtcggcat atgtcacacg cttgttcaat actactcagc
      541 agcttcatac tagtacctcc acctccacct ccaccacctc ctccgctaga tgagctcagc
      601 cgacctatag ctctgactgc gtgacctgtc ttattccact tacgacgtga tgagtcgacc
      661 atggtggcga gatccttatc gtcatcgtcg tacagatccc gacccatttg ctgtccacca
      721 gtcatgctag ccataccatg atgatgatga tgatgagaac ccatggtgga tctgttaacg
      781 aattgatccc ccgatctgtt aacgaattcc caattcccta ttcagagttc tcttcttgta
      841 ttcaataatt acttcttggc agatttcagt agttgcagtt gatt
//
