LOCUS       sequence-111609-         204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes six a1 operator sites upstream of minimal pCYC1 promoter 
            driving yeast enhanced BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 204)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: 6x_a1gRNAOp_pCYC1m_yeBFP2
FEATURES             Location/Qualifiers
     source          1..204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8..24
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggtact
       61 gtatgtacat acagtaggat cctcgaaaat attaattact gtatgtacat acagtaggat
      121 cctcgaaaat attaattact gtatgtacat acagtaggat cctcgaaaat attaattact
      181 gtatgtacat acagtaggat cctc
//
