LOCUS       sequence-111610-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pattB-nsyb-M13::GAL4DBDo
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..84
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(88..96,97..111)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             139..195
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             238..678
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 tcatcgacag ccgaattcac catgggttct catcatcatc atcatcatgg tatggctagc
       61 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatctcgcc
      121 accatggtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt cagagctata
      181 ggtcggctga gctcatctag cggaggaggt ggtggaggtg gaggtggagg tactagtatg
      241 aagctgctga gtagtattga acaagcgtgt gacatatgcc gacttaagaa gctgaagtgc
      301 agcaaggaaa agccgaagtg cgccaaatgt cttaagaaca attgggagtg ccggtactcg
      361 ccaaagacga agcgaagccc tttgacacgc gctcatttga ctgaagtgga gagtcgcctg
      421 gagcgccttg agcagttgtt cctcctcatt tttccccgtg aagacctcga catgatactg
      481 aagatggata gtctccaaga tattaaggcc ctgctcactg gcctgtttgt tcaggataac
      541 gtcaacaagg atgccgtgac ggaccgcctt gcctcggtgg agacagatat gccacttacc
      601 ttgcggcagc atcgcatctc ggctaccagc agtagtgaag aaagttccaa taagggtcaa
      661 cggcaactga cggtatcgta agacgtctat cgataccgtc gactaaagcc aaatagaaaa
      721 ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc
      781 aa
//
