LOCUS       sequence-111611-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes twelve a1 operator sites upstream of minimal pCYC1 promoter 
            driving yeast enhanced BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: 12x_a1gRNAOp_pCYC1m_yeBFP2
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagcttgata tcgaattcct gcagcccggg tactgtatgt acatacagta ggatcctcga
       61 aaatattaat tactgtatgt acatacagta ggatcctcga aaatattaat tactgtatgt
      121 acatacagta ggatcctcga aaatattaat tactgtatgt acatacagta ggatcctcga
      181 aaatattaat tactgtatgt acatacagta ggatcctcga aaatattaat tactgtatgt
      241 acatacagta ggatcctact gtatgtacat acagtaatta atattttcga ggatcctact
      301 gtatgtacat acagtaatta atattttcga ggatcctact gtatgtacat acagtaatta
      361 atattttcga ggatcctact gtatgtacat acagtaatta atattttcga ggatcctact
      421 gtatgtacat acagtaatta atattttcga ggatcctact gtatgtacat acagtaatta
      481 atattttcga ggatcctatg gcatgcatgt gctctgtatg tatataaaac tcttgttttc
      541 ttcttttctc taaatattct ttccttatac attaggacct ttgcagcata aattactata
      601 cttctataga cacacaaaca caaatacaca cactaatcta gataaaaaat ggtttccaag
//
