LOCUS       sequence-111612-         298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes twelve a1 operator sites upstream of minimal pCYC1 promoter 
            driving yeast enhanced BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: 12x_a1gRNAOp_pCYC1m_yeBFP2
FEATURES             Location/Qualifiers
     source          1..298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..38
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     23..39
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattgggtc cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc
       61 tgcagcccgg gtactgtatg tacatacagt aggatcctcg aaaatattaa ttactgtatg
      121 tacatacagt aggatcctcg aaaatattaa ttactgtatg tacatacagt aggatcctcg
      181 aaaatattaa ttactgtatg tacatacagt aggatcctcg aaaatattaa ttactgtatg
      241 tacatacagt aggatcctcg aaaatattaa ttactgtatg tacatacagt aggatcct
//
