LOCUS       sequence-111614-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pUASTattB-MKII::nlsLexADBDo
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(105..461,462..713))
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             complement(723..743)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
       61 gaagtaaggt tccttcacaa agatcctcta gaggtaccct cgagttacag ccaatctccg
      121 ttgcggatca cacccacggc caagccctcg atagtaaagg actgctgtcg cagatccacc
      181 acgatcggct tgaactcgct attctccggc agcagctcga ccttattgcc ttgcttcttg
      241 agacgtttca cagtgacctc atcgtctatc ctggcaacga ctacctggcc gttcctgaca
      301 tcctgtgtct tatggacggc gaggaggtca ccatccataa taccgatgtc cttcatggac
      361 attcccgaca cccgcaacag gaaatcagca tttggcttaa acaaggaggg gtccacctga
      421 tagtgtccct ctatgtgttg ctgggccagc agcggctcgc cagcggccac gcgtcccacc
      481 agtggcaggc cctcttcctc ctcctgcaac agccggattc cgcggctcgc accggacacg
      541 atttcaatga cacccttgcg ggccagtgcc ttcaagtgct cctccgcggc gttaggcgat
      601 cgaaagccca gtcgctgtgc tatctctgcg cgggtcggag gcatacccgt ttgggatata
      661 tgatcccgta tcaaatcaaa aacttcctgt tgtcgggccg tgagagcctt cattggatct
      721 tctacttttc gcttcttctt gggtggacta gtacctccac ctccacctcc accacctcct
      781 ccgctagccg aaaaatttct ggtcgccaac attgtcgtaa gtatggctcc ctttagcttg
      841 cgccgcgcat taaacatggt gagatctgtt aacgaattga tcc
//
